REGULATED INFORMATION Publication relating to transparency notifications Mont-Saint-Guibert (Belgium), October 3, 2025, 10:30 pm CET / 4:30 pm ET – In accordance with article 14 of the Act of 2 May 2007 on the disclosure of large shareholdings, Nyxoah SA (Euronext Brussels/Nasdaq: NYXH) announces that it received a transparency notification as detailed below. Vestal Point Capital On October 1, 2025, Nyxoah received a transparency notification from Vestal Point Capital following an acquisition or disposal of voting securities or voting rights. Based on the notification, Vestal Point Capital holds 1,113,575 voting rights, representing 2.97% of the total number of voting rights on September 24, 2025 (37,544,782). The notification dated September 30, 2025 contains the following information: Reason for the notification: Acquisition or disposal of voting securities or voting rights Downward crossing of the lowest threshold Notification by: a parent undertaking or a controlling person Persons subject to the notification requirement: Vestal Point Capital, LLC (with address at 632 Broadway, Suite 602, New York, NY 10012, USA) Vestal Point Capital, LP (with address at 632 Broadway, Suite 602, New York, NY 10012, USA) Ryan Wilder (with address at 632 Broadway, Suite 602, New York, NY 10012, USA) Date on which the threshold was crossed: September 24, 2025 Threshold that is crossed: 3% Denominator: 37,544,782 Notified details: A) Voting rights Previous notification After the transaction # of voting rights # of voting rights % of voting rights Holders of voting rights Linked to securities Not linked to the securities Linked to securities Not linked to the securities Ryan Wilder 0 0 0 0.00% 0.00% Vestal Point Capital, LLC 0 0 0 0.00% 0.00% Vestal Point Capital, LP 1,809,843 1,113,575 0 2.97% 0.00% Subtotal 1,809,843 1,113,575 2.97% TOTAL 1,113,575 2.97% Full chain of controlled undertakings through which the holding is effectively held: Vestal Point Capital, LP (investment manager) is controlled by Vestal Point Capital, LLC (general